This workshop, to be hosted in the afternoon of Bioinformatics Open Days 2020, is an introduction to differential gene expression analysis of medium-sized single-cell RNA-seq (scRNA-seq) obtained using the 10x Genomics Chromium System. Participants will learn about the basic concepts behind the technology, its applications and concepts, and go through a hands-on exercise where they will perform a re-analysis of a sample of ~3,000 cells from the Mouse Cell Atlas (MCA). For this they will make use of a user-friendly web application built on top of the popular Seurat package, that allows interactive exploration of the concepts involved in the analysis, including filtering, normalization, dimensionality reduction, clustering, and marker gene identification.
Attendance requires registration in the BOD 2020 workshops. Register until February 14th through the following link.
Universidade do Minho, Campus de Gualtar
R. da Universidade